Bioprocess Engineering
© TU Berlin/PR/Felix Noak

Model based Scale-Down experiments for a convertible high throughput screening framework

Project summary

Small-scale high-throughput systems like the integrated robotic mini bioreactor platform at the Chair of Bioprocess Engineering (Haby et al., 2019) offer promising potential for characterising and discriminating a wide range of strains quickly and cost-effectively. However, the upscale process of bioprocess development shows a weak robustness to reproduce results from such systems on a production scale due to different technical influences.
Oscillatory control strategies such as pulse-based feeding can help to overcome this problem as they can mimic the gradients in larger reactors that significantly affect organism performance (Anane et al., 2018). In addition, Anane shows that mechanistic models allow a dynamic description of these gradients and accordingly the estimation of physiological parameters.
Building on his study, this project will develop an efficient applicability for Mini BioReactors (MBR). Here, the physiological parameters act as a key for the scale down by considering them as a comparative key figure within the different scales. Based on these new metrics, a system will be developed to condition the MBR control to a specific scale. The next step is to transform screening from a static to a dynamic method. The extrapolating property of a model is used to perform strain-specific screening with adaptive process control (Hans et al., 2020) in pre-defined boundary conditions.
This project will enable a faster, cheaper, more robust and optimised approach to strain discrimination in bioprocess development with relation to production scale.

Project partner:
WACKER Biotech

Project duration:
08/2020 - current

Project funding:
Contract researche

Responsible person:
Sebastian Hans
Fabian Schröder

    © TU Berlin/PR/Felix Noak

    The Work Group

    This project is part of the High-Throughput Bioprocess Development (HTBD). Read more about our group, our mission and related projects.

    © Dominic Simon

    Opportunity / Thesis

    Are you looking for a challenging thesis? We often have open topics! Send us your interests and strengths together with a short CV via email.

    © TU Berlin/BVT/S.Hans

    The Lab

    Interested in the lab? The HTBD laboratory is a leader in the application of automated and model-based bioprocess control. Take a look at your liquid handling stations, mini bioreactor system and other lab equipment in our virtual tour.

    To the Lab
    © Philipp Arnoldt


    The HTBD - Groupe offers various lectures dedicated to the basics of our work. You can find an overview of our current courses here.