Bioprocess Engineering
© TU Berlin/PR/Felix Noak

Systems biology platform for the creation of lean-proteome Escherichia coli strains

In nature Escherichia coli has applied complex strategies to adopt to its environment. This includes a wide range of genes and proteins which are necessary to survive under rough conditions. In industrial processes such harsh conditions are eliminated and the production of many native proteins could be considered as unnecessary. In the LEANPROT project such proteins will be identified and removed from the genome of Escherichia coli. This will be done in an iterative process of protein identification, modelling and biological experiments.

In a highly automated screening process the chair of bioprocess engineering assume the screening process and the biological experiments. For this purpose the automation platform will be used. Aim of the TU Berlin part is to shorten the time and experiments of the screening using model-based experimental design, automated cultivations and sample analyse.

Project partner

CCFFT - Competence Center of Food and Fermentation Technologies, Estonia
Latvia University of Agriculture, Latvia
NTNU - Norwegian University of Science and Technology, Norway
biotechrabbit GmbH, Germany

Project duration
12/2015 - 05/2019

Project funding

Project staff

Dr. Mario Nicolas Cruz-Bouranzou
Dr. Florian Glauche
PhD: Sebastian Hans

© TU Berlin/PR/Felix Noak

The Work Group

This project is part of the High-Throughput Bioprocess Development (HTBD). Read more about our group, our mission and related projects.

© Dominic Simon

Opportunity / Thesis

Are you looking for a challenging thesis? We often have open topics! Send us your interests and strengths together with a short CV via email.

© TU Berlin/BVT/S.Hans

The Lab

Interested in the lab? The HTBD laboratory is a leader in the application of automated and model-based bioprocess control. Take a look at your liquid handling stations, mini bioreactor system and other lab equipment in our virtual tour.

To the Lab
© Philipp Arnoldt


The HTBD - Groupe offers various lectures dedicated to the basics of our work. You can find an overview of our current courses here.