Bioprocess Engineering
Bioprocess Engineering

Publications

2021

2020

F. Glauche, M. N. C. Bournazou, A. Knepper, M. Föllmer, S. Junne, and P. Neubauer, “Design of Experiments for Fed-Batch Process Development in Shaken Cultures,” p. 3.

S. Hans, B. Haby, N. Krausch, T. Barz, P. Neubauer, and M. N. Cruz-Bournazou, “Automated Conditional Screening of Multiple Escherichia coli Strains in Parallel Adaptive Fed-Batch Cultivations,” Bioengineering, vol. 7, no. 4, p. 145, Nov. 2020, doi: 10.3390/bioengineering7040145.

V. Lyubenova, M. Ignatova, O. Roeva, S. Junne, and P. Neubauer, “Adaptive Monitoring of Biotechnological Processes Kinetics,” Processes, vol. 8, no. 10, p. 1307, Oct. 2020, doi: 10.3390/pr8101307.

F. Kaspar, R. T. Giessmann, S. Westarp, K. F. Hellendahl, N. Krausch, I. Thiele, M. C. Walczak, P. Neubauer, and A. Wagner, “Spectral Unmixing‐Based Reaction Monitoring of Transformations between Nucleosides and Nucleobases,” ChemBioChem, vol. 21, no. 18, pp. 2604–2610, Sep. 2020, doi: 10.1002/cbic.202000204.

D. Gómez-Ríos, V. A. López-Agudelo, H. Ramírez-Malule, P. Neubauer, S. Junne, S. Ochoa, and R. Ríos-Estepa, “A Genome-Scale Insight into the Effect of Shear Stress During the Fed-Batch Production of Clavulanic Acid by Streptomyces Clavuligerus,” Microorganisms, vol. 8, no. 9, p. 1255, Sep. 2020, doi: 10.3390/microorganisms8091255.

C. E. Gómez-Camacho, K. Pellicer Alborch, A. Bockisch, P. Neubauer, S. Junne, and B. Ruggeri, “Monitoring the Physiological State in the Dark Fermentation of Maize/Grass Silage Using Flow Cytometry and Electrooptic Polarizability Measurements,” Bioenerg. Res., Aug. 2020, doi: 10.1007/s12155-020-10184-x.

F. Nejati, S. Junne, J. Kurreck, and P. Neubauer, “Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR,” Front. Microbiol., vol. 11, p. 1291, Jun. 2020, doi: 10.3389/fmicb.2020.01291.

S. Hans, C. Ulmer, H. Narayanan, T. Brautaset, N. Krausch, P. Neubauer, I. Schäffl, M. Sokolov, and M. N. Cruz Bournazou, “Monitoring Parallel Robotic Cultivations with Online Multivariate Analysis,” Processes, vol. 8, no. 5, p. 582, May 2020, doi: 10.3390/pr8050582.

L. Theuer, J. Randek, S. Junne, P. Neubauer, C.-F. Mandenius, and V. Beni, “Single-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoring,” Processes, vol. 8, no. 3, p. 321, Mar. 2020, doi: 10.3390/pr8030321.

H. Yehia, S. Westarp, V. Röhrs, F. Kaspar, R. T. Giessmann, H. F. T. Klare, K. Paulick, P. Neubauer, J. Kurreck, and A. Wagner, “Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations,” Molecules, vol. 25, no. 4, p. 934, Feb. 2020, doi: 10.3390/molecules25040934.

F. Nejati, S. Junne, and P. Neubauer, “A Big World in Small Grain: A Review of Natural Milk Kefir Starters,” Microorganisms, vol. 8, no. 2, p. 192, Feb. 2020, doi: 10.3390/microorganisms8020192.

T. Menzel, P. Neubauer, and S. Junne, “Role of Microbial Hydrolysis in Anaerobic Digestion,” Energies, vol. 13, no. 21, p. 5555, Jan. 2020, doi: 10.3390/en13215555.

S. L. Riedel and S. Junne, “Bioökonomie: Aus Resten schöpfen,” Nachrichten aus der Chemie, vol. 68, no. 11, pp. 38–40, 2020, doi: https://doi.org/10.1002/nadc.20204095432.

V. Meyer and R. Rapp, Mind the Fungi. 2020.

N. Krausch, S. Hans, F. Fiedler, S. Lucia, P. Neubauer, and M. N. Cruz Bournazou, “From Screening to Production: a Holistic Approach of High-throughput Model-based Screening for Recombinant Protein Production,” in Computer Aided Chemical Engineering, vol. 48, Elsevier, 2020, pp. 1723–1728. doi: 10.1016/B978-0-12-823377-1.50288-3.

2019

A. M. Marbà-Ardébol, J. Emmerich, M. Muthig, P. Neubauer, and S. Junne, “In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses,” JoVE, no. 154, p. 57823, Dec. 2019, doi: 10.3791/57823.

B. Haby, S. Hans, E. Anane, A. Sawatzki, N. Krausch, P. Neubauer, and M. N. Cruz Bournazou, “Integrated Robotic Mini Bioreactor Platform for Automated, Parallel Microbial Cultivation With Online Data Handling and Process Control,” SLAS TECHNOLOGY: Translating Life Sciences Innovation, vol. 24, no. 6, pp. 569–582, Dec. 2019, doi: 10.1177/2472630319860775.

D. Gómez-Ríos, S. Junne, P. Neubauer, S. Ochoa, R. Ríos-Estepa, and H. Ramírez-Malule, “Characterization of the Metabolic Response of Streptomyces clavuligerus to Shear Stress in Stirred Tanks and Single-Use 2D Rocking Motion Bioreactors for Clavulanic Acid Production,” Antibiotics, vol. 8, no. 4, p. 168, Dec. 2019, doi: 10.3390/antibiotics8040168.

A. Gawin, K. Peebo, S. Hans, H. Ertesvåg, M. Irla, P. Neubauer, and T. Brautaset, “Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production,” Microb Cell Fact, vol. 18, no. 1, p. 80, Dec. 2019, doi: 10.1186/s12934-019-1128-7.

E. Anane, Á. C. García, B. Haby, S. Hans, N. Krausch, M. Krewinkel, P. Hauptmann, P. Neubauer, and M. N. Cruz Bournazou, “A model‐based framework for parallel scale‐down fed‐batch cultivations in mini‐bioreactors for accelerated phenotyping,” Biotechnology and Bioengineering, vol. 116, no. 11, pp. 2906–2918, Nov. 2019, doi: 10.1002/bit.27116.

F. Kaspar, R. T. Giessmann, N. Krausch, P. Neubauer, A. Wagner, and M. Gimpel, “A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases,” MPs, vol. 2, no. 3, p. 60, Jul. 2019, doi: 10.3390/mps2030060.

R. T. Giessmann, N. Krausch, F. Kaspar, M. N. Cruz Bournazou, A. Wagner, P. Neubauer, and M. Gimpel, “Dynamic Modelling of Phosphorolytic Cleavage Catalyzed by Pyrimidine-Nucleoside Phosphorylase,” Processes, vol. 7, no. 6, p. 380, Jun. 2019, doi: 10.3390/pr7060380.

N. Krausch, T. Barz, A. Sawatzki, M. Gruber, S. Kamel, P. Neubauer, and M. N. Cruz Bournazou, “Monte Carlo Simulations for the Analysis of Non-linear Parameter Confidence Intervals in Optimal Experimental Design,” Front. Bioeng. Biotechnol., vol. 7, p. 122, May 2019, doi: 10.3389/fbioe.2019.00122.

R. Spann, J. Glibstrup, K. Pellicer-Alborch, S. Junne, P. Neubauer, C. Roca, D. Kold, A. E. Lantz, G. Sin, K. V. Gernaey, and U. Krühne, “CFD predicted pH gradients in lactic acid bacteria cultivations: SPANN et al.,” Biotechnology and Bioengineering, vol. 116, no. 4, pp. 769–780, Apr. 2019, doi: 10.1002/bit.26868.

D. Gómez-Ríos, H. Ramírez-Malule, P. Neubauer, S. Junne, and R. Ríos-Estepa, “Data of clavulanic acid and clavulanate-imidazole stability at low temperatures,” Data in Brief, vol. 23, p. 103775, Apr. 2019, doi: 10.1016/j.dib.2019.103775.

D. Gómez-Ríos, H. Ramírez-Malule, P. Neubauer, S. Junne, and R. Ríos-Estepa, “Degradation Kinetics of Clavulanic Acid in Fermentation Broths at Low Temperatures,” Antibiotics, vol. 8, no. 1, p. 6, Jan. 2019, doi: 10.3390/antibiotics8010006.

J. Emmerich, Q. Tang, Y. Wang, P. Neubauer, S. Junne, and S. Maaß, “Optical inline analysis and monitoring of particle size and shape distributions for multiple applications: Scientific and industrial relevance,” Chinese Journal of Chemical Engineering, vol. 27, no. 2, 2019, doi: 10.1016/j.cjche.2018.11.011.

M. Conrady, A. Lemoine, M. H. Limberg, M. Oldiges, P. Neubauer, and S. Junne, “Carboxylic acid consumption and production by Corynebacterium glutamicum,” Biotechnology Progress, vol. 35, no. 3, p. e2804, 2019, doi: https://doi.org/10.1002/btpr.2804.

A. Bockisch, E. Kielhorn, P. Neubauer, and S. Junne, “Process analytical technologies to monitor the liquid phase of anaerobic cultures,” Process Biochemistry, vol. 76, 2019, doi: 10.1016/j.procbio.2018.10.005.

2018

A. Sawatzki, S. Hans, H. Narayanan, B. Haby, N. Krausch, M. Sokolov, F. Glauche, S. Riedel, P. Neubauer, and M. Cruz Bournazou, “Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform,” Bioengineering, vol. 5, no. 4, p. 101, Nov. 2018, doi: 10.3390/bioengineering5040101.

F. Delvigne, B. Zacchetti, P. Fickers, B. Fifani, F. Roulling, C. Lefebvre, P. Neubauer, and S. Junne, “Improving control in microbial cell factories: from single-cell to large-scale bioproduction,” FEMS microbiology letters, vol. 365, no. 22, Sep. 2018, doi: 10.1093/femsle/fny236.

S. Hans, M. Gimpel, F. Glauche, P. Neubauer, and M. Cruz-Bournazou, “Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates,” Microorganisms, vol. 6, no. 3, p. 60, Jun. 2018, doi: 10.3390/microorganisms6030060.

H. Yehia, S. Kamel, K. Paulick, P. Neubauer, and A. Wagner, “Substrate Spectra of Nucleoside Phosphorylases and their Potential in the Production of Pharmaceutically Active Compounds,” CPD, vol. 23, no. 45, pp. 6913–6935, Feb. 2018, doi: 10.2174/1381612823666171024155811.

H. Ramirez-Malule, S. Junne, M. Nicolás Cruz-Bournazou, P. Neubauer, and R. Ríos-Estepa, “Streptomyces clavuligerus shows a strong association between TCA cycle intermediate accumulation and clavulanic acid biosynthesis,” Applied Microbiology and Biotechnology, vol. 102, no. 9, pp. 4009–4023, 2018, doi: 10.1007/s00253-018-8841-8.

K. Pellicer-Alborch, A. Angersbach, P. Neubauer, and S. Junne, “Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification of Lactobacillus plantarum Cultures,” Frontiers in Bioengineering and Biotechnology, vol. 6, no. 188, pp. 1–10, 2018, doi: 10.3389/fbioe.2018.00188.

A. M. Marbà-Ardébol, A. Bockisch, P. Neubauer, and S. Junne, “Sterol synthesis and cell size distribution under oscillatory growth conditions in Saccharomyces cerevisiae scale-down cultivations,” Yeast, vol. 35, no. 2, pp. 213–223, 2018, doi: 10.1002/yea.3281.

A.-M. A. M. Marbà-Ardébol, J. Emmerich, M. Muthig, P. Neubauer, and S. Junne, “Real-time monitoring of the budding index in Saccharomyces cerevisiae batch cultivations with in situ microscopy,” Microbial Cell Factories, vol. 17, no. 1, 2018, doi: 10.1186/s12934-018-0922-y.

T. Kurt, A. Maria, M. Ardébol, Z. Turan, P. Neubauer, S. Junne, and V. Meyer, “Rocking Aspergillus : morphology ‑ controlled cultivation of Aspergillus niger in a wave ‑ mixed bioreactor for the production of secondary metabolites,” Microbial Cell Factories, pp. 1–9, 2018, doi: 10.1186/s12934-018-0975-y.

M. Kögler, A. Paul, E. Anane, M. Birkholz, A. Bunker, T. Viitala, M. Maiwald, S. Junne, and P. Neubauer, “Comparison of time-gated surface-enhanced raman spectroscopy (TG-SERS) and classical SERS based monitoring of Escherichia coli cultivation samples,” Biotechnology Progress, 2018, doi: 10.1002/btpr.2665.

S. Junne and P. Neubauer, “How scalable and suitable are single-use bioreactors?,” Current Opinion in Biotechnology, vol. 53, pp. 240–247, 2018, doi: 10.1016/j.copbio.2018.04.003.

E. Brand, S. Junne, E. Anane, M. N. M. N. Cruz-Bournazou, and P. Neubauer, “Importance of the cultivation history for the response of Escherichia coli to oscillations in scale-down experiments,” Bioprocess and Biosystems Engineering, vol. 41, no. 9, pp. 1305–1313, 2018, doi: 10.1007/s00449-018-1958-4.

2017

2016

P. Neubauer and S. Junne, “Scale-Up and Scale-Down Methodologies for Bioreactors,” in Bioreactors: Design, Operation and Novel Applications, C.-F. Mandenius, Ed. Weinheim: Wiley-VCH Verlag GmbH, 2016, pp. 323–354. doi: 10.1002/9783527683369.ch11.

H. Ramirez-Malule, A. Restrepo, W. Cardona, S. Junne, P. Neubauer, and R. Rios-Estepa, “Inversion of the stereochemical configuration (3S, 5S)-clavaminic acid into (3R, 5R)-clavulanic acid: A computationally-assisted approach based on experimental evidence,” Journal of Theoretical Biology, vol. 395, pp. 40–50, 2016, doi: 10.1016/j.jtbi.2016.01.028.

H. Ramirez-Malule, S. Junne, C. López, J. Zapata, A. Sáez, P. Neubauer, and R. Rios-Estepa, “An improved HPLC-DAD method for clavulanic acid quantification in fermentation broths of Streptomyces clavuligerus,” Journal of Pharmaceutical and Biomedical Analysis, vol. 120, pp. 241–247, 2016, doi: 10.1016/j.jpba.2015.12.035.

N. M. G. Oosterhuis and S. Junne, “Design, Applications, and Development of Single-Use Bioreactors,” in Bioreactors, 2016, pp. 261–294. doi: 10.1002/9783527683369.ch9.

P. Neubauer and S. Junne, “Robuste industrielle Bioprozesse: vom Labor- zum Industriemaßstab und zurück,” BioSpektrum, vol. 22, no. 2, pp. 204–207, 2016, doi: 10.1007/s12268-016-0672-5.

A. M. Marba-Ardebol, X. Turon, P. Neubauer, S. Junne, A. Marba Ardebol, X. Turon, P. Neubauer, and S. Junne, “Application of flow cytometry analysis to elucidate the impact of scale-down conditions in Escherichia coli cultivations,” Afinidad, vol. 73, no. 573, pp. 7–15, 2016.

A. Lemoine, M. H. M. H. Limberg, S. Kästner, M. Oldiges, P. Neubauer, and S. Junne, “Performance loss of Corynebacterium glutamicum cultivations under scale-down conditions using complex media,” Engineering in Life Sciences, vol. 16, no. 7, pp. 620–632, 2016, doi: 10.1002/elsc.201500144.

S. Junne, A. M. Marbà-Ardébol, and P. Neubauer, “Neue Applikationsfelder für Single-use-Bioreaktoren,” BioSpektrum, vol. 22, no. 1, 2016, doi: 10.1007/s12268-016-0660-9.

F. C. Ertem, J. Martínez-Blanco, M. Finkbeiner, P. Neubauer, and S. Junne, “Life cycle assessment of flexibly fed biogas processes for an improved demand-oriented biogas supply,” Bioresource Technology, vol. 219, 2016, doi: 10.1016/j.biortech.2016.07.123.

2015

F. Glauche, G. T. John, S. Arain, A. Knepper, A. Neubauer, D. Goelling, C. Lang, N. Violet, R. King, and P. Neubauer, “Toward Microbioreactor Arrays: A Slow-Responding Oxygen Sensor for Monitoring of Microbial Cultures in Standard 96-Well Plates,” J Lab Autom., vol. 20, no. 4, pp. 438–446, Aug. 2015, doi: 10.1177/2211068215573924.

S. Sachse, A. Bockisch, U. Enseleit, F. Gerlach, K. Ahlborn, T. Kuhnke, U. Rother, E. Kielhorn, P. Neubauer, S. Junne, and W. Vonau, “On the use of electrochemical multi-sensors in biologically charged media,” Journal of Sensors and Sensor Systems, vol. 4, no. 2, pp. 295–303, 2015, doi: 10.5194/jsss-4-295-2015.

A. Lemoine, N. Maya Martnez-Iturralde, R. Spann, P. Neubauer, and S. Junne, “Response of Corynebacterium glutamicum exposed to oscillating cultivation conditions in a two- and a novel three-compartment scale-down bioreactor,” Biotechnology and Bioengineering, vol. 112, no. 6, pp. 1220–1231, 2015, doi: 10.1002/bit.25543.

A. Lemoine, S. Junne, and P. Neubauer, “Scale-down studies of corynebacterium glutamicum cultivations for a consistent bioprocess development,” in New Trends in Corynebacterium glutamicum: Beyond the Amino Acids, 2015.

E. Kielhorn, S. Sachse, M. Moench-Tegeder, H.-J. Naegele, C. Haelsig, H. Oechsner, W. Vonau, P. Neubauer, and S. Junne, “Multiposition Sensor Technology and Lance-Based Sampling for Improved Monitoring of the Liquid Phase in Biogas Processes,” Energy and Fuels, vol. 29, no. 7, 2015, doi: 10.1021/ef502816c.

2014

F. Glauche, G. T. John, S. Arain, A. Knepper, A. Neubauer, D. Goelling, C. Lang, N. Violet, R. King, and P. Neubauer, “Toward Microbioreactor Arrays: A Slow-Responding Oxygen Sensor for Monitoring of Microbial Cultures in Standard 96-Well Plates,” J Lab Autom., vol. 20, no. 4, pp. 438–446, Aug. 2015, doi: 10.1177/2211068215573924.

S. Sachse, A. Bockisch, U. Enseleit, F. Gerlach, K. Ahlborn, T. Kuhnke, U. Rother, E. Kielhorn, P. Neubauer, S. Junne, and W. Vonau, “On the use of electrochemical multi-sensors in biologically charged media,” Journal of Sensors and Sensor Systems, vol. 4, no. 2, pp. 295–303, 2015, doi: 10.5194/jsss-4-295-2015.

A. Lemoine, N. Maya Martnez-Iturralde, R. Spann, P. Neubauer, and S. Junne, “Response of Corynebacterium glutamicum exposed to oscillating cultivation conditions in a two- and a novel three-compartment scale-down bioreactor,” Biotechnology and Bioengineering, vol. 112, no. 6, pp. 1220–1231, 2015, doi: 10.1002/bit.25543.

A. Lemoine, S. Junne, and P. Neubauer, “Scale-down studies of corynebacterium glutamicum cultivations for a consistent bioprocess development,” in New Trends in Corynebacterium glutamicum: Beyond the Amino Acids, 2015.

E. Kielhorn, S. Sachse, M. Moench-Tegeder, H.-J. Naegele, C. Haelsig, H. Oechsner, W. Vonau, P. Neubauer, and S. Junne, “Multiposition Sensor Technology and Lance-Based Sampling for Improved Monitoring of the Liquid Phase in Biogas Processes,” Energy and Fuels, vol. 29, no. 7, 2015, doi: 10.1021/ef502816c.

2013

N. M. Oosterhuis, P. Neubauer, and S. Junne, “Single-use bioreactors for microbial cultivation,” Pharmaceutical Bioprocessing, vol. 1, no. 2, pp. 167–177, 2013, doi: 10.4155/pbp.13.19.

N. Oosterhuis and S. Junne, “E. coli cultivation in a 12L and 120L CELL-tainerTM single-use bioreactor,” BioProcess International, vol. 11, no. 7, 2013.

P. Neubauer, N. Cruz, F. Glauche, S. Junne, A. Knepper, and M. Raven, “Consistent development of bioprocesses from microliter cultures to the industrial scale,” Engineering in Life Sciences, vol. 13, no. 3, pp. 224–238, 2013, doi: 10.1002/elsc.201200021.

V. Lyubenova, S. Junne, M. Ignatova, and P. Neubauer, “Software sensor design considering oscillating conditions as present in industrial scale fed-batch cultivations,” Biotechnology and Bioengineering, vol. 110, no. 7, 2013, doi: 10.1002/bit.24870.

S. Junne, T. Solymosi, N. Oosterhuis, and P. Neubauer, Cultivation of cells and microorganisms in wave-mixed disposable bag bioreactors at different scales, vol. 85. 2013. doi: 10.1002/cite.201200149.

F. Hillig, S. Annemüller, M. Chmielewska, M. Pilarek, S. Junne, and P. Neubauer, “Bioprocess development in single-use systems for heterotrophic marine microalgae,” Chemie-Ingenieur-Technik, vol. 85, no. 1–2, pp. 153–161, 2013, doi: 10.1002/cite.201200143.

2012

2011

S. Junne, A. Klingner, J. Kabisch, T. Schweder, and P. Neubauer, “A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: Impact of oscillations on Bacillus subtilis fed-batch cultivations,” Biotechnology Journal, vol. 6, no. 8, pp. 1009–1017, 2011, doi: 10.1002/biot.201100293.

J. Glazyrina, E. Materne, F. Hillig, P. Neubauer, and S. Junne, “Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidation,” Biotechnology Journal, vol. 6, no. 8, pp. 1003–1008, 2011, doi: 10.1002/biot.201100281.

2010

P. Neubauer and S. Junne, Scale-down simulators for metabolic analysis of large-scale bioprocesses, vol. 21. 2010. doi: 10.1016/j.copbio.2010.02.001.

S. Junne, M. Nicolas Cruz-Bournazou, A. Angersbach, and P. Götz, “Electrooptical monitoring of cell polarizability and cell size in aerobic Escherichia coli batch cultivations,” Journal of Industrial Microbiology and Biotechnology, vol. 37, no. 9, 2010, doi: 10.1007/s10295-010-0742-5.

J. Glazyrina, E.-M. Materne, T. Dreher, D. Storm, S. Junne, T. Adams, G. Greller, and P. Neubauer, “High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor,” Microbial Cell Factories, vol. 9, 2010, doi: 10.1186/1475-2859-9-42.

2000 - 2009

M. N. C. Bournazou, H. Arellano-Garcia, J. C. Schöneberger, S. Junne, P. Neubauer, and G. Woznya, A novel approach to mechanism recognition in escherichia coli fed-batch fermentations, vol. 27. 2009. doi: 10.1016/S1570-7946(09)70329-3.

G. Melzer, S. Junne, R. Wohlgemuth, D. C. Hempel, and P. Götz, “Production of epoxide hydrolases in batch fermentations of Botryosphaeria rhodina,” Journal of Industrial Microbiology and Biotechnology, vol. 35, no. 6, 2008, doi: 10.1007/s10295-008-0306-0.

S. Junne, E. Klein, A. Angersbach, and P. Goetz, “Electrooptical measurements for monitoring metabolite fluxes in acetone-butanol-ethanol fermentations,” Biotechnology and Bioengineering, vol. 99, no. 4, 2008, doi: 10.1002/bit.21639.

J. Glazyrina, S. Junne, P. Thiesen, K. Lunkenheimer, and P. Goetz, “In situ removal and purification of biosurfactants by automated surface enrichment,” Applied Microbiology and Biotechnology, vol. 81, no. 1, 2008, doi: 10.1007/s00253-008-1620-1.

J. Maczek, S. Junne, P. Nowak, and P. Goetz, “Metabolic flux analysis of the sterol pathway in the yeast Saccharomyces cerevisiae,” Bioprocess and Biosystems Engineering, vol. 29, no. 4, pp. 241–252, 2006, doi: 10.1007/s00449-006-0072-1.

B. Fahnert, H. Lilie, and P. Neubauer, “Inclusion bodies: formation and utilisation,” Adv Biochem Eng Biotechnol, vol. 89, pp. 93–142, 2004, doi: 10.1007/b93995.

M. Los, G. Wegrzyn, and P. Neubauer, “A role for bacteriophage T4 rI gene function in the control of phage development during pseudolysogeny and in slowly growing host cells,” Res Microbiol, vol. 154, no. 8, pp. 547–52, Oct. 2003, doi: 10.1016/S0923-2508(03)00151-7.

P. Neubauer, H. Y. Lin, and B. Mathiszik, “Metabolic load of recombinant protein production: inhibition of cellular capacities for glucose uptake and respiration after induction of a heterologous gene in Escherichia coli,” Biotechnol Bioeng, vol. 83, no. 1, pp. 53–64, Jul. 2003, doi: 10.1002/bit.10645.

J. Rautio, K. B. Barken, J. Lahdenpera, A. Breitenstein, S. Molin, and P. Neubauer, “Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates,” Microb Cell Fact, vol. 2, no. 1, p. 4, Apr. 2003, doi: 10.1186/1475-2859-2-4.

M. I. Viitanen, A. Vasala, P. Neubauer, and T. Alatossava, “Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli,” Microb Cell Fact, vol. 2, no. 1, p. 2, Apr. 2003, doi: 10.1186/1475-2859-2-2.

A. M. Sanden et al., “Limiting factors in Escherichia coli fed-batch production of recombinant proteins,” Biotechnol Bioeng, vol. 81, no. 2, pp. 158–66, Jan. 2003, doi: 10.1002/bit.10457.

S. B. Tummala, S. G. Junne, C. J. Paredes, and E. T. Papoutsakis, “Transcriptional Analysis of Product-Concentration Driven Changes in Cellular Programs of Recombinant Clostridium acetobutylicum Strains,” Biotechnology and Bioengineering, vol. 84, no. 7, 2003, doi: 10.1002/bit.10851.

S. B. Tummala, S. G. Junne, and E. T. Papoutsakis, “Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations,” Journal of Bacteriology, vol. 185, no. 12, 2003, doi: 10.1128/JB.185.12.3644-3653.2003.

A. Potamitou, P. Neubauer, A. Holmgren, and A. Vlamis-Gardikas, “Expression of Escherichia coli glutaredoxin 2 is mainly regulated by ppGpp and sigmaS,” J Biol Chem, vol. 277, no. 20, pp. 17775–80, May 2002, doi: 10.1074/jbc.M201306200.

T. Schweder, H. Y. Lin, B. Jurgen, A. Breitenstein, S. Riemschneider, V. Khalameyzer, A. Gupta, K. Buttner, and P. Neubauer, “Role of the general stress response during strong overexpression of a heterologous gene in Escherichia coli,” Appl Microbiol Biotechnol, vol. 58, no. 3, pp. 330–7, Mar. 2002, doi: 10.1007/s00253-001-0904-5.

J. Winter, P. Neubauer, R. Glockshuber, and R. Rudolph, “Increased production of human proinsulin in the periplasmic space of Escherichia coli by fusion to DsbA,” J Biotechnol, vol. 84, no. 2, pp. 175–85, Nov. 2001, doi: 10.1016/s0168-1656(00)00356-4.

H. Y. Lin, B. Mathiszik, B. Xu, S. O. Enfors, and P. Neubauer, “Determination of the maximum specific uptake capacities for glucose and oxygen in glucose-limited fed-batch cultivations of Escherichia coli,” Biotechnol Bioeng, vol. 73, no. 5, pp. 347–57, Jun. 2001, doi: 10.1002/bit.1068.

S. O. Enfors et al., “Physiological responses to mixing in large scale bioreactors,” J Biotechnol, vol. 85, no. 2, pp. 175–85, Feb. 2001, doi: 10.1016/s0168-1656(00)00365-5.

B. Jurgen, H. Y. Lin, S. Riemschneider, C. Scharf, P. Neubauer, R. Schmid, M. Hecker, and T. Schweder, “Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fed-batch fermentations,” Biotechnol Bioeng, vol. 70, no. 2, pp. 217–24, Oct. 2000, doi: 10.1002/1097-0290(20001020)70:2<217::aid-bit11>3.0.co;2-w.

H. Y. Lin and P. Neubauer, “Influence of controlled glucose oscillations on a fed-batch process of recombinant Escherichia coli,” J Biotechnol, vol. 79, no. 1, pp. 27–37, Apr. 2000, doi: 10.1016/s0168-1656(00)00217-0.

vor 2000

M. Slominska, P. Neubauer, and G. Wegrzyn, “Regulation of bacteriophage lambda development by guanosine 5’-diphosphate-3’-diphosphate,” Virology, vol. 262, no. 2, pp. 431–41, Sep. 1999, doi: 10.1006/viro.1999.9907.

S. Meyer, N. Noisommit-Rizzi, M. Reuss, and P. Neubauer, “Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli,” Anal Biochem, vol. 271, no. 1, pp. 43–52, Jun. 1999, doi: 10.1006/abio.1999.4119.

A. Teich, S. Meyer, H. Y. Lin, L. Andersson, S. Enfors, and P. Neubauer, “Growth rate related concentration changes of the starvation response regulators sigmaS and ppGpp in glucose-limited fed-batch and continuous cultures of Escherichia coli,” Biotechnol Prog, vol. 15, no. 1, pp. 123–9, Jan. 1999, doi: 10.1021/bp980102h.

D. Fischer, A. Teich, P. Neubauer, and R. Hengge-Aronis, “The general stress sigma factor sigmaS of Escherichia coli is induced during diauxic shift from glucose to lactose,” J Bacteriol, vol. 180, no. 23, pp. 6203–6, Dec. 1998.

A. Teich, H. Y. Lin, L. Andersson, S. Meyer, and P. Neubauer, “Amplification of ColE1 related plasmids in recombinant cultures of Escherichia coli after IPTG induction,” J Biotechnol, vol. 64, no. 2–3, pp. 197–210, Oct. 1998, doi: 10.1016/s0168-1656(98)00108-4.

P. Neubauer and K. Hofmann, “Efficient use of lactose for the lac promoter-controlled overexpression of the main antigenic protein of the foot and mouth disease virus in Escherichia coli under fed-batch fermentation conditions,” FEMS Microbiol Rev, vol. 14, no. 1, pp. 99–102, May 1994, doi: 10.1111/j.1574-6976.1994.tb00080.x.

T. Schweder, I. Schmidt, H. Herrmann, P. Neubauer, M. Hecker, and K. Hofmann, “An expression vector system providing plasmid stability and conditional suicide of plasmid-containing cells,” Appl Microbiol Biotechnol, vol. 38, no. 1, pp. 91–3, Oct. 1992, doi: 10.1007/BF00169425.

P. Neubauer, K. Hofmann, O. Holst, B. Mattiasson, and P. Kruschke, “Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as inducer,” Appl Microbiol Biotechnol, vol. 36, no. 6, pp. 739–44, Mar. 1992, doi: 10.1007/BF00172185.

G. Wegrzyn, P. Neubauer, S. Krueger, M. Hecker, and K. Taylor, “Stringent control of replication of plasmids derived from coliphage lambda,” Mol Gen Genet, vol. 225, no. 1, pp. 94–8, Jan. 1991, doi: 10.1007/BF00282646.

K. H. Hofmann, P. Neubauer, S. Riethdorf, and M. Hecker, “Amplification of pBR322 plasmid DNA in Escherichia coli relA strains during batch and fed-batch fermentation,” J Basic Microbiol, vol. 30, no. 1, pp. 37–41, 1990, doi: 10.1002/jobm.3620300111.