Biokatalyse
Prof. Dr. Matthias Höhne
| Einrichtung | Biokatalyse/ Biologische Chemie |
|---|---|
| Gebäude | L |
| Raum | L 101 |
Wissenschaftlicher Werdegang
| Seit 02/2023 | Professor für Biokatalyse / Biologische Chemie | Technische Universität Berlin |
| 2018 - 2023 | Gruppenleiter am Institut für Biochemie | Universität Greifswald |
| 2012 - 2018 | Gruppenleiter am Institut für Biochemie | Universität Greifswald |
| 2009 - 2011 | Postdoc bei Prof. Dr. A. Jäschke am Institut für Pharmazie und molekulare Biotechnologie (IPMB) | Universität Heidelberg |
| 2005 - 2009 | Promotion bei Prof. U. Bornscheuer, Thema: Synthese optisch aktiver Amine mit ω-Transaminasen | Universität Greifswald |
| 2005 | Aufenthalt bei Prof. K. Hult, Thema: Moleküldynamische Simulation | KTH Stockholm, Schweden |
| 2000 - 2005 | Studium Diplom Biochemie | Universität Greifswald |
Forschungsprojekte
| Photo-Biokatalyse und Enzymkaskaden | |||
| Enzyme discovery | |||
| Protein engineering (ERC Starting grant) | |||
| Struktur-Funktionsbeziehungen von Enzymen und Binde-/Transportproteinen |
Preise & Auszeichnungen
2019 Greifswald University Club Junior Award für anwendungsnahe Forschung
Bücher
2018
Protein Engineering: Methods and Protocols
In Bornscheuer, Uwe T. and Höhne, Matthias, Editor, Band 1685 aus Methods in Molecular Biology
Herausgeber: Springer New York, New York, NY
1st edition 2018 Edition
2018
- ISBN
- 9781493973668
Publikationen
2022
Kollipara,
Manideep;
Matzel,
Philipp;
Bornscheuer,
Uwe;
Höhne,
Matthias
Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity
Chemie Ingenieur Technik, 94 (11) :1836–1844
2022
ISSN: 0009-286X
Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity
Chemie Ingenieur Technik, 94 (11) :1836–1844
2022
ISSN: 0009-286X
Heinks,
Tobias;
Paulus,
Jannik;
Koopmeiners,
Simon;
Beuel,
Tobias;
Sewald,
Norbert;
Höhne,
Matthias;
Bornscheuer,
Uwe T.;
Mollard,
Gabriele
Recombinant l-Amino Acid Oxidase with Broad Substrate Spectrum for Co-substrate Recycling in (S)-Selective Transaminase-Catalyzed Kinetic Resolutions
Chembiochem : a European journal of chemical biology, 23 (16) :e202200329
2022
Recombinant l-Amino Acid Oxidase with Broad Substrate Spectrum for Co-substrate Recycling in (S)-Selective Transaminase-Catalyzed Kinetic Resolutions
Chembiochem : a European journal of chemical biology, 23 (16) :e202200329
2022
Kollipara,
Manideep;
Matzel,
Philipp;
Sowa,
Miriam;
Brott,
Stefan;
Bornscheuer,
Uwe;
Höhne,
Matthias
Characterization of proteins from the 3N5M family reveals an operationally stable amine transaminase
Applied microbiology and biotechnology, 106 (17) :5563–5574
2022
Characterization of proteins from the 3N5M family reveals an operationally stable amine transaminase
Applied microbiology and biotechnology, 106 (17) :5563–5574
2022
Janson,
Nils;
Heinks,
Tobias;
Beuel,
Tobias;
Alam,
Sarfaraz;
Höhne,
Matthias;
Bornscheuer,
Uwe T.;
Mollard,
Gabriele;
Sewald,
Norbert
Efficient Site–Selective Immobilization of Aldehyde–Tagged Peptides and Proteins by Knoevenagel Ligation
ChemCatChem, 14 (2)
2022
ISSN: 1867-3899
Efficient Site–Selective Immobilization of Aldehyde–Tagged Peptides and Proteins by Knoevenagel Ligation
ChemCatChem, 14 (2)
2022
ISSN: 1867-3899
2021
Yi,
Dong;
Bayer,
Thomas;
Badenhorst,
Christoffel P. S.;
Wu,
Shuke;
Doerr,
Mark;
Höhne,
Matthias;
Bornscheuer,
Uwe T.
Recent trends in biocatalysis
Chemical Society reviews, 50 (14) :8003–8049
2021
Recent trends in biocatalysis
Chemical Society reviews, 50 (14) :8003–8049
2021
2020
Marx,
Lisa;
Ríos-Lombardía,
Nicolás;
Süss,
Philipp;
Höhne,
Matthias;
Morís,
Francisco;
González-Sabín,
Javier;
Berglund,
Per
Chemoenzymatic Synthesis of Sertraline
European Journal of Organic Chemistry, 2020 (4) :510–513
2020
ISSN: 1434193X
Chemoenzymatic Synthesis of Sertraline
European Journal of Organic Chemistry, 2020 (4) :510–513
2020
ISSN: 1434193X
Voss,
Moritz;
Xiang,
Chao;
Esque,
Jérémy;
Nobili,
Alberto;
Menke,
Marian J.;
André,
Isabelle;
Höhne,
Matthias;
Bornscheuer,
Uwe T.
Creation of (R)-Amine Transaminase Activity within an \textgreeka-Amino Acid Transaminase Scaffold
ACS chemical biology, 15 (2) :416–424
2020
Creation of (R)-Amine Transaminase Activity within an \textgreeka-Amino Acid Transaminase Scaffold
ACS chemical biology, 15 (2) :416–424
2020
Matassa,
Claudia;
Romani,
Alessandra;
Ormerod,
Dominic;
Bornscheuer,
Uwe T.;
Höhne,
Matthias;
Satyawali,
Yamini
JeffaminecircledR ED–600: a polyether amine donor for enzymatic transamination in organic solvent/solvent–free medium with membrane–assisted product extraction
Journal of Chemical Technology & Biotechnology, 95 (3) :604–613
2020
ISSN: 0268-2575
JeffaminecircledR ED–600: a polyether amine donor for enzymatic transamination in organic solvent/solvent–free medium with membrane–assisted product extraction
Journal of Chemical Technology & Biotechnology, 95 (3) :604–613
2020
ISSN: 0268-2575
Matassa,
Claudia;
Ormerod,
Dominic;
Bornscheuer,
Uwe T.;
Höhne,
Matthias;
Satyawali,
Yamini
Three–liquid–phase Spinning Reactor for the Transaminase–catalyzed Synthesis and Recovery of a Chiral Amine
ChemCatChem, 12 (5) :1288–1291
2020
ISSN: 1867-3899
Three–liquid–phase Spinning Reactor for the Transaminase–catalyzed Synthesis and Recovery of a Chiral Amine
ChemCatChem, 12 (5) :1288–1291
2020
ISSN: 1867-3899
2019
Sviatenko,
Olha;
Ríos–Lombardía,
Nicolás;
Morís,
Francisco;
González–Sabín,
Javier;
Venkata Manideep,
Kollipara;
Merdivan,
Simon;
Günther,
Sebastian;
Süss,
Philipp;
Höhne,
Matthias
One–pot Synthesis of 4–Aminocyclohexanol Isomers by Combining a Keto Reductase and an Amine Transaminase
ChemCatChem, 11 (23) :5794–5799
2019
ISSN: 1867-3899
One–pot Synthesis of 4–Aminocyclohexanol Isomers by Combining a Keto Reductase and an Amine Transaminase
ChemCatChem, 11 (23) :5794–5799
2019
ISSN: 1867-3899
Matzel,
Philipp;
Wenske,
Sebastian;
Merdivan,
Simon;
Günther,
Sebastian;
Höhne,
Matthias
Synthesis of \textgreekb–Chiral Amines by Dynamic Kinetic Resolution of \textgreeka–Branched Aldehydes Applying Imine Reductases
ChemCatChem, 11 (17) :4281–4285
2019
ISSN: 1867-3899
Synthesis of \textgreekb–Chiral Amines by Dynamic Kinetic Resolution of \textgreeka–Branched Aldehydes Applying Imine Reductases
ChemCatChem, 11 (17) :4281–4285
2019
ISSN: 1867-3899
Schwendenwein,
Daniel;
Ressmann,
Anna K.;
Doerr,
Mark;
Höhne,
Matthias;
Bornscheuer,
Uwe T.;
Mihovilovic,
Marko D.;
Rudroff,
Florian;
Winkler,
Margit
Random Mutagenesis–Driven Improvement of Carboxylate Reductase Activity using an Amino Benzamidoxime–Mediated High–Throughput Assay
Advanced Synthesis & Catalysis
2019
ISSN: 1615-4150
Random Mutagenesis–Driven Improvement of Carboxylate Reductase Activity using an Amino Benzamidoxime–Mediated High–Throughput Assay
Advanced Synthesis & Catalysis
2019
ISSN: 1615-4150
Höhne,
Matthias
Engineering imine reductases for industrial applications
Nature Catalysis, 2 (10) :841–842
2019
Engineering imine reductases for industrial applications
Nature Catalysis, 2 (10) :841–842
2019
Zhang,
Wuyuan;
Fueyo,
Elena Fernandez;
Hollmann,
Frank;
Martin,
Laura Leemans;
Pesic,
Milja;
Wardenga,
Rainer;
Höhne,
Matthias;
Schmidt,
Sandy
Combining Photo-Organo Redox- and Enzyme Catalysis Facilitates Asymmetric C-H Bond Functionalization
European Journal of Organic Chemistry, 2019 (1) :80–84
2019
ISSN: 1434193X
Combining Photo-Organo Redox- and Enzyme Catalysis Facilitates Asymmetric C-H Bond Functionalization
European Journal of Organic Chemistry, 2019 (1) :80–84
2019
ISSN: 1434193X
Matassa,
Claudia;
Ormerod,
Dominic;
Bornscheuer,
Uwe T.;
Höhne,
Matthias;
Satyawali,
Yamini
Application of novel High Molecular Weight amine donors in chiral amine synthesis facilitates integrated downstream processing and provides in situ product recovery opportunities
Process Biochemistry, 80 :17–25
2019
ISSN: 13595113
Application of novel High Molecular Weight amine donors in chiral amine synthesis facilitates integrated downstream processing and provides in situ product recovery opportunities
Process Biochemistry, 80 :17–25
2019
ISSN: 13595113
Mystkowska,
Agata Anna;
Robb,
Craig;
Vidal-Melgosa,
Silvia;
Vanni,
Chiara;
Fernandez-Guerra,
Antonio;
Höhne,
Matthias;
Hehemann,
Jan-Hendrik
Molecular recognition of the beta-glucans laminarin and pustulan by a SusD-like glycan-binding protein of a marine Bacteroidetes
The FEBS journal, 285 (23) :4465–4481
2018
Molecular recognition of the beta-glucans laminarin and pustulan by a SusD-like glycan-binding protein of a marine Bacteroidetes
The FEBS journal, 285 (23) :4465–4481
2018
Voss M,
Höhne M
A quantum mechanics based engineering approach to improve transaminases for the conversion of bulky substrates
ACS Catal, 2018 (8) :11524–11533
2018
A quantum mechanics based engineering approach to improve transaminases for the conversion of bulky substrates
ACS Catal, 2018 (8) :11524–11533
2018
2017
Calvelage,
Sten;
Dörr,
Mark;
Höhne,
Matthias;
Bornscheuer,
Uwe T.
A Systematic Analysis of the Substrate Scope of ( S )- and ( R )-Selective Amine Transaminases
Advanced Synthesis & Catalysis, 359 (23) :4235–4243
2017
ISSN: 1615-4150
A Systematic Analysis of the Substrate Scope of ( S )- and ( R )-Selective Amine Transaminases
Advanced Synthesis & Catalysis, 359 (23) :4235–4243
2017
ISSN: 1615-4150
Matzel,
P.;
Gand,
M.;
Höhne,
M.
One-step asymmetric synthesis of (R)- and (S)-rasagiline by reductive amination applying imine reductases
Green Chemistry, 19 (2) :385–389
2017
ISSN: 1463-9262
One-step asymmetric synthesis of (R)- and (S)-rasagiline by reductive amination applying imine reductases
Green Chemistry, 19 (2) :385–389
2017
ISSN: 1463-9262
Matzel,
Philipp;
Krautschick,
Lukas;
Höhne,
Matthias
Photometric Characterization of the Reductive Amination Scope of the Imine Reductases from Streptomyces tsukubaensis and Streptomyces ipomoeae
Chembiochem : a European journal of chemical biology, 18 (20) :2022–2027
2017
Photometric Characterization of the Reductive Amination Scope of the Imine Reductases from Streptomyces tsukubaensis and Streptomyces ipomoeae
Chembiochem : a European journal of chemical biology, 18 (20) :2022–2027
2017
2016
Gand,
Martin;
Thöle,
Christian;
Müller,
Hubertus;
Brundiek,
Henrike;
Bashiri,
Ghader;
Höhne,
Matthias
A NADH-accepting imine reductase variant: Immobilization and cofactor regeneration by oxidative deamination
Journal of biotechnology, 230 :11–18
2016
A NADH-accepting imine reductase variant: Immobilization and cofactor regeneration by oxidative deamination
Journal of biotechnology, 230 :11–18
2016
Wetzl,
Dennis;
Gand,
Martin;
Ross,
Alfred;
Müller,
Hubertus;
Matzel,
Philipp;
Hanlon,
Steven P.;
Müller,
Michael;
Wirz,
Beat;
Höhne,
Matthias;
Iding,
Hans
Asymmetric Reductive Amination of Ketones Catalyzed by Imine Reductases
ChemCatChem, 8 (12) :2023–2026
2016
ISSN: 1867-3899
Asymmetric Reductive Amination of Ketones Catalyzed by Imine Reductases
ChemCatChem, 8 (12) :2023–2026
2016
ISSN: 1867-3899
Steffen-Munsberg,
Fabian;
Matzel,
Philipp;
Sowa,
Miriam A.;
Berglund,
Per;
Bornscheuer,
Uwe T.;
Höhne,
Matthias
Bacillus anthracis \textgreekw-amino acid:pyruvate transaminase employs a different mechanism for dual substrate recognition than other amine transaminases
Applied microbiology and biotechnology, 100 (10) :4511–4521
2016
Bacillus anthracis \textgreekw-amino acid:pyruvate transaminase employs a different mechanism for dual substrate recognition than other amine transaminases
Applied microbiology and biotechnology, 100 (10) :4511–4521
2016
Höhne,
Matthias;
Kabisch,
Johannes
Brewing Painkillers: A Yeast Cell Factory for the Production of Opioids from Sugar
Angewandte Chemie (International ed. in English), 55 (4) :1248–1250
2016
Brewing Painkillers: A Yeast Cell Factory for the Production of Opioids from Sugar
Angewandte Chemie (International ed. in English), 55 (4) :1248–1250
2016
Cuetos,
Anibal;
Steffen-Munsberg,
Fabian;
Mangas Sanchez,
Juan;
Frese,
Amina;
Bornscheuer,
Uwe T.;
Höhne,
Matthias;
Grogan,
Gideon
Structural Basis for Phospholyase Activity of a Class III Transaminase Homologue
Chembiochem : a European journal of chemical biology, 17 (24) :2308–2311
2016
Structural Basis for Phospholyase Activity of a Class III Transaminase Homologue
Chembiochem : a European journal of chemical biology, 17 (24) :2308–2311
2016