Prof. Dr. Matthias Höhne

Prof. Dr.

Matthias Höhne

FGL Strukturprofessur


Einrichtung Biokatalyse/ Biologische Chemie
Sekretariat L 1
Gebäude L
Raum L 101

Wissenschaftlicher Werdegang

Seit 02/2023Professor für Biokatalyse / Biologische ChemieTechnische Universität Berlin
2018 - 2023Gruppenleiter am Institut für BiochemieUniversität Greifswald
2012 - 2018Gruppenleiter am Institut für BiochemieUniversität Greifswald
2009 - 2011Postdoc bei Prof. Dr. A. Jäschke am Institut für Pharmazie und molekulare Biotechnologie (IPMB)Universität Heidelberg
2005 - 2009Promotion bei Prof. U. Bornscheuer, Thema: Synthese optisch aktiver Amine mit ω-TransaminasenUniversität Greifswald
2005Aufenthalt bei Prof. K. Hult, Thema: Moleküldynamische SimulationKTH Stockholm, Schweden
2000 - 2005Studium Diplom BiochemieUniversität Greifswald


Photo-Biokatalyse und Enzymkaskaden   
Enzyme discovery   
Protein engineering (ERC Starting grant)   
Struktur-Funktionsbeziehungen von Enzymen und Binde-/Transportproteinen   

Preise & Auszeichnungen

2019  Greifswald University Club Junior Award für anwendungsnahe Forschung



Protein Engineering: Methods and Protocols
In Bornscheuer, Uwe T. and Höhne, Matthias, Editor, Band 1685 aus Methods in Molecular Biology
Herausgeber: Springer New York, New York, NY
1st edition 2018 Edition



Kollipara, Manideep; Matzel, Philipp; Bornscheuer, Uwe; Höhne, Matthias
Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity
Chemie Ingenieur Technik, 94 (11) :1836–1844
ISSN: 0009-286X
Heinks, Tobias; Paulus, Jannik; Koopmeiners, Simon; Beuel, Tobias; Sewald, Norbert; Höhne, Matthias; Bornscheuer, Uwe T.; Mollard, Gabriele
Recombinant l-Amino Acid Oxidase with Broad Substrate Spectrum for Co-substrate Recycling in (S)-Selective Transaminase-Catalyzed Kinetic Resolutions
Chembiochem : a European journal of chemical biology, 23 (16) :e202200329
Kollipara, Manideep; Matzel, Philipp; Sowa, Miriam; Brott, Stefan; Bornscheuer, Uwe; Höhne, Matthias
Characterization of proteins from the 3N5M family reveals an operationally stable amine transaminase
Applied microbiology and biotechnology, 106 (17) :5563–5574
Janson, Nils; Heinks, Tobias; Beuel, Tobias; Alam, Sarfaraz; Höhne, Matthias; Bornscheuer, Uwe T.; Mollard, Gabriele; Sewald, Norbert
Efficient Site–Selective Immobilization of Aldehyde–Tagged Peptides and Proteins by Knoevenagel Ligation
ChemCatChem, 14 (2)
ISSN: 1867-3899


Yi, Dong; Bayer, Thomas; Badenhorst, Christoffel P. S.; Wu, Shuke; Doerr, Mark; Höhne, Matthias; Bornscheuer, Uwe T.
Recent trends in biocatalysis
Chemical Society reviews, 50 (14) :8003–8049


Marx, Lisa; Ríos-Lombardía, Nicolás; Süss, Philipp; Höhne, Matthias; Morís, Francisco; González-Sabín, Javier; Berglund, Per
Chemoenzymatic Synthesis of Sertraline
European Journal of Organic Chemistry, 2020 (4) :510–513
ISSN: 1434193X
Voss, Moritz; Xiang, Chao; Esque, Jérémy; Nobili, Alberto; Menke, Marian J.; André, Isabelle; Höhne, Matthias; Bornscheuer, Uwe T.
Creation of (R)-Amine Transaminase Activity within an \textgreeka-Amino Acid Transaminase Scaffold
ACS chemical biology, 15 (2) :416–424
Matassa, Claudia; Romani, Alessandra; Ormerod, Dominic; Bornscheuer, Uwe T.; Höhne, Matthias; Satyawali, Yamini
JeffaminecircledR ED–600: a polyether amine donor for enzymatic transamination in organic solvent/solvent–free medium with membrane–assisted product extraction
Journal of Chemical Technology & Biotechnology, 95 (3) :604–613
ISSN: 0268-2575
Matassa, Claudia; Ormerod, Dominic; Bornscheuer, Uwe T.; Höhne, Matthias; Satyawali, Yamini
Three–liquid–phase Spinning Reactor for the Transaminase–catalyzed Synthesis and Recovery of a Chiral Amine
ChemCatChem, 12 (5) :1288–1291
ISSN: 1867-3899


Sviatenko, Olha; Ríos–Lombardía, Nicolás; Morís, Francisco; González–Sabín, Javier; Venkata Manideep, Kollipara; Merdivan, Simon; Günther, Sebastian; Süss, Philipp; Höhne, Matthias
One–pot Synthesis of 4–Aminocyclohexanol Isomers by Combining a Keto Reductase and an Amine Transaminase
ChemCatChem, 11 (23) :5794–5799
ISSN: 1867-3899
Matzel, Philipp; Wenske, Sebastian; Merdivan, Simon; Günther, Sebastian; Höhne, Matthias
Synthesis of \textgreekb–Chiral Amines by Dynamic Kinetic Resolution of \textgreeka–Branched Aldehydes Applying Imine Reductases
ChemCatChem, 11 (17) :4281–4285
ISSN: 1867-3899
Schwendenwein, Daniel; Ressmann, Anna K.; Doerr, Mark; Höhne, Matthias; Bornscheuer, Uwe T.; Mihovilovic, Marko D.; Rudroff, Florian; Winkler, Margit
Random Mutagenesis–Driven Improvement of Carboxylate Reductase Activity using an Amino Benzamidoxime–Mediated High–Throughput Assay
Advanced Synthesis & Catalysis
ISSN: 1615-4150
Höhne, Matthias
Engineering imine reductases for industrial applications
Nature Catalysis, 2 (10) :841–842
Zhang, Wuyuan; Fueyo, Elena Fernandez; Hollmann, Frank; Martin, Laura Leemans; Pesic, Milja; Wardenga, Rainer; Höhne, Matthias; Schmidt, Sandy
Combining Photo-Organo Redox- and Enzyme Catalysis Facilitates Asymmetric C-H Bond Functionalization
European Journal of Organic Chemistry, 2019 (1) :80–84
ISSN: 1434193X
Matassa, Claudia; Ormerod, Dominic; Bornscheuer, Uwe T.; Höhne, Matthias; Satyawali, Yamini
Application of novel High Molecular Weight amine donors in chiral amine synthesis facilitates integrated downstream processing and provides in situ product recovery opportunities
Process Biochemistry, 80 :17–25
ISSN: 13595113


Mystkowska, Agata Anna; Robb, Craig; Vidal-Melgosa, Silvia; Vanni, Chiara; Fernandez-Guerra, Antonio; Höhne, Matthias; Hehemann, Jan-Hendrik
Molecular recognition of the beta-glucans laminarin and pustulan by a SusD-like glycan-binding protein of a marine Bacteroidetes
The FEBS journal, 285 (23) :4465–4481
Voss M, Höhne M
A quantum mechanics based engineering approach to improve transaminases for the conversion of bulky substrates
ACS Catal, 2018 (8) :11524–11533


Calvelage, Sten; Dörr, Mark; Höhne, Matthias; Bornscheuer, Uwe T.
A Systematic Analysis of the Substrate Scope of ( S )- and ( R )-Selective Amine Transaminases
Advanced Synthesis & Catalysis, 359 (23) :4235–4243
ISSN: 1615-4150
Matzel, P.; Gand, M.; Höhne, M.
One-step asymmetric synthesis of (R)- and (S)-rasagiline by reductive amination applying imine reductases
Green Chemistry, 19 (2) :385–389
ISSN: 1463-9262
Matzel, Philipp; Krautschick, Lukas; Höhne, Matthias
Photometric Characterization of the Reductive Amination Scope of the Imine Reductases from Streptomyces tsukubaensis and Streptomyces ipomoeae
Chembiochem : a European journal of chemical biology, 18 (20) :2022–2027


Gand, Martin; Thöle, Christian; Müller, Hubertus; Brundiek, Henrike; Bashiri, Ghader; Höhne, Matthias
A NADH-accepting imine reductase variant: Immobilization and cofactor regeneration by oxidative deamination
Journal of biotechnology, 230 :11–18
Wetzl, Dennis; Gand, Martin; Ross, Alfred; Müller, Hubertus; Matzel, Philipp; Hanlon, Steven P.; Müller, Michael; Wirz, Beat; Höhne, Matthias; Iding, Hans
Asymmetric Reductive Amination of Ketones Catalyzed by Imine Reductases
ChemCatChem, 8 (12) :2023–2026
ISSN: 1867-3899
Steffen-Munsberg, Fabian; Matzel, Philipp; Sowa, Miriam A.; Berglund, Per; Bornscheuer, Uwe T.; Höhne, Matthias
Bacillus anthracis \textgreekw-amino acid:pyruvate transaminase employs a different mechanism for dual substrate recognition than other amine transaminases
Applied microbiology and biotechnology, 100 (10) :4511–4521
Höhne, Matthias; Kabisch, Johannes
Brewing Painkillers: A Yeast Cell Factory for the Production of Opioids from Sugar
Angewandte Chemie (International ed. in English), 55 (4) :1248–1250
Cuetos, Anibal; Steffen-Munsberg, Fabian; Mangas Sanchez, Juan; Frese, Amina; Bornscheuer, Uwe T.; Höhne, Matthias; Grogan, Gideon
Structural Basis for Phospholyase Activity of a Class III Transaminase Homologue
Chembiochem : a European journal of chemical biology, 17 (24) :2308–2311