Biokatalyse
Prof. Dr. Nediljko Budisa
Prof. Dr.
Nediljko Budisa
FGL Strukturprofessur
| Einrichtung | Biokatalyse |
|---|---|
| Sekretariat | L1 |
| Gebäude | L |
| Raum | L 161 |
| Adresse | Müller-Breslau-Str. 10 10623 Berlin |
Wissenschaftlicher Werdegang
| 2010 - bis dato |
| Professor und Lehrstuhlinhaber für Biokatalyse am Institut für Chemie der Technischen Universität Berlin. |
| 2006 - 2010 |
| Privatdozent für Biochemie und Biotechnologie im Fachbereich 'Chemical Protein Engineering' an der Technischen Universität München. |
| 2005 - 2010 |
| Nachwuchsgruppenleiter im Fachbereich Molekulare Biotechnologie am Max Planck Institut für Biochemie, Technischen Universität München. |
| 2001 - 2005 |
| Wissenschaftllicher Mitarbeiter und Gruppenleiter im Fachbereich 'Protein Engineering' am Max Planck Institut für Biochemie, Habilitation in Biochemie, Technische Universität München. |
| 1999 - 2000 |
| Beteiligung an der Gründung der Firma Proteros Biostructures GmbH |
| 1997 - 1999 |
| Postdoktorand der Abteilung für Strukturelle Forschung (R. Huber) am Max Planck Institut für Biochemie, Technische Universität München. |
| 1993 - 1997 |
| Erlangung der Doktorwürde mit summa cum laude unter Anleitung von Prof. Dr. R. Huber, am Max Planck Institut für Biochemie, Technische Universität München. |
| 1989 - 1993 |
| Studium der Molekularen Biologie und Molekularen Biophysik an der Universität in Zagreb. |
| 1986 - 1990 |
| Lehramtsstudium in den Fächern Chemie und Biologie an der Universität in Zagreb. |
Forschungsinteressen und wissenschaftliche Leistungen
| Größte Errungenschaft: |
| Einer der Gründer und Pionier im Forschungsbereich des "genetic code engineering". |
| Visionen: |
| Genomweite Neugestaltung des genetischen Codes als vielversprechendster Weg zur Änderung und Erweiterung der grundlegenden Chemie des Lebens (d.h., Erschaffung von synthetischem Leben). |
| Forschungsziel: |
| Experimentelle Änderung des genetischen Codes. |
| Wissenschaftliche Interessen: |
| Synthetische Biologie & Xenobiologie; Bioorthogonale Chemie und klassischer Genetik (Stamm-Engineering und Evolution); Entwicklung von Plattformen mit erweitertem genetischem Code; Herstellung maßgeschneiderter Peptide, Proteine und Proteome; De novo Protein- und Proteomedesign, durch die Verknüpfung von gerichteter Enzymevolution; metabolisches Engineering; Synthetische Chemie; Herstellung von genetisch kodierbaren Wirkstoffen und Biomaterialien. |
Ehrungen, Auszeichnungen und Funktionen in wiss. Beiräten u. Beratergremien
2014: Einer der Gründer und führender Vertreter im Bereich der Xenobiologie.
2013: Gründer des ersten Berliner iGEM-Teams im Bereich der synthetischen Biologie. (Goldmedaille 2014)
2012: Mitglied im Redaktionsbeirat der SynBio-Fachzeitschrift „Symplectic Biology“2011Mitglied der ERA-Net ERASynBio Advisory Board.
Mitglied der BBAW Arbeitsgruppe „Gentechnologiebericht” für Synthetische Biologie.
Mitglied des COST Netzwerks.
2010: Mitglied des Steering Committee der Max-Planck-Gesellschaft für Synthetische Biologie.
2008: Mitglied der Arbeitsgruppe “Synthetic and Systems Biology” von DECHEMA, Frankfurt; Mitglied des strategischen Beratungsgremium (ab 2012).
2007: Mitglied des Exzellencluster CIPSM der Ludwig Maximilian Universität /Technischen Universität München (2007 – 2010).
2004: Gutachter und Berater für BMBF2004BioFuture-Nachwuchspreis des BMBF
2001: Prüfer des Graduiertenkollegs IMPRS am Max-Planck Institut für Biochemie, München.
2001: Gründer und Mitglied der Deutschen Gesellschaft für Proteomforschung1998„BioRegio"-Auszeichnung für Biotechnologie - Unternehmensgründung „Proteros Biostructures"
Publikationen
2019
Diwo,
C.;
Budisa,
N.
Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation
Genes, 2019 (10) :e17
2019
Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation
Genes, 2019 (10) :e17
2019
Baumann,
T.;
Hauf,
M.;
Schildhauer,
F.;
Eberl,
K.B.;
Durkin,
P.M.;
Deniz,
E.;
Löffler,
J.G.;
Acevedo-Rocha,
C.G.;
Jaric,
J.;
Martins,
B.M.;
Dobbek,
H.;
Bredenbeck,
J.;
Budisa,
N.
Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater
Angew. Chem. Int. Ed. Engl., 58 (9) :2899-2903
2019
Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater
Angew. Chem. Int. Ed. Engl., 58 (9) :2899-2903
2019
Kubyshkin,
V.;
Budisa,
N.
Anticipating alien cells with alternative genetic codes: away from the alanine world!
Curr. Opin. Biotechnol., 2019 (60) :242-249
2019
Anticipating alien cells with alternative genetic codes: away from the alanine world!
Curr. Opin. Biotechnol., 2019 (60) :242-249
2019
Gerrits,
M.;
Merk,
H.;
Budisa,
N.
Site-Specific Chemoselective Pyrrolysine Analogues Incorporation Using the Cell-Free Protein Synthesis System
ACS Synth. Biol., 2019 (8(2)) :381-390
2019
Site-Specific Chemoselective Pyrrolysine Analogues Incorporation Using the Cell-Free Protein Synthesis System
ACS Synth. Biol., 2019 (8(2)) :381-390
2019
Schneider,
T.;
Gavrilova,
I.;
Budisa,
N.
Synthesis of a new metal chelating amino acid: Terpyridyl-alanine
Tetrahedron Lett., 2018 (18) :2053-2063
2019
Synthesis of a new metal chelating amino acid: Terpyridyl-alanine
Tetrahedron Lett., 2018 (18) :2053-2063
2019
Kubyshkin,
V.;
Budisa,
N.
Promotion of the collagen triple helix in a hydrophobic environment
Org. Biomol. Chem., 17 (19) :2502-2507
2019
Promotion of the collagen triple helix in a hydrophobic environment
Org. Biomol. Chem., 17 (19) :2502-2507
2019
Nojoumi,
S.;
Ying,
M.;
Schwagerus,
S.;
Hackenberger,
C.P.R.;
Budisa,
N.
In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation
Int. J. Mol. Sci., 20(9= (e2299)
2019
In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation
Int. J. Mol. Sci., 20(9= (e2299)
2019
Schneider,
T.;
Budisa,
N.
Expanding the DOPA Universe with Genetically Encoded, Mussel-Inspired Bioadhesives for Material Sciences and Medicine
ChemBioChem, 2019 (Mar 4. Epub)
2019
Expanding the DOPA Universe with Genetically Encoded, Mussel-Inspired Bioadhesives for Material Sciences and Medicine
ChemBioChem, 2019 (Mar 4. Epub)
2019
Baumann,
T.;
Hauf,
M.;
Richter,
F.;
Albers,
S.;
Möglich,
A.;
Ignatova,
Z.;
Budisa,
N.
Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine
Int. J. Mol. Sci., 20 (9) :e2343
2019
Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine
Int. J. Mol. Sci., 20 (9) :e2343
2019
2018
Budisa,
N.;
Fehse,
B.;
Müller-Röber,
B.;
Reich,
J.;
Walter,
J.
Looking back and forth: Development and current challenges in various core areas of genetic engineering
Accounting for a High Technology - Fourth Genetic Technology Report of Interdisciplinary Research Group of the Berlin-Brandenburg Academy of Sciences and Humanities, 2018 :203-236
2018
Herausgeber: Nomos Verlag
ISSN: 978-3-8452-9379-0
Looking back and forth: Development and current challenges in various core areas of genetic engineering
Accounting for a High Technology - Fourth Genetic Technology Report of Interdisciplinary Research Group of the Berlin-Brandenburg Academy of Sciences and Humanities, 2018 :203-236
2018
Herausgeber: Nomos Verlag
ISSN: 978-3-8452-9379-0
- ISBN
- 978-3-8487-5183-9
Kubyshkin,
V.;
Grage,
S. L.;
Bürck,
J.;
Ulrich,
A. S.;
Budisa,
N.
Transmembrane Polyproline Helix
J. Phys. Chem. Lett, 9 :2170-2174
2018
Transmembrane Polyproline Helix
J. Phys. Chem. Lett, 9 :2170-2174
2018
Synthetic biology – metabolic engineering
In Zhao, Huimin and Zeng, A.-P, Editor, Band 162 aus Advances in biochemical engineering/biotechnology
Herausgeber: Springer, Cham, Switzerland
2018
- ISBN
- 9783319553184
Schneider,
T.;
Kubyshkin,
V.;
Budisa,
N.
Synthesis of a Photo‐Caged DOPA Derivative by Selective Alkylation of 3, 4‐Dihydroxybenzaldehyde
Eur. J. Org. Chem., 2018(18) :2053-2063
2018
Synthesis of a Photo‐Caged DOPA Derivative by Selective Alkylation of 3, 4‐Dihydroxybenzaldehyde
Eur. J. Org. Chem., 2018(18) :2053-2063
2018
Ma,
Y.;
Salvo,
M. L.;
Budisa,
N.
Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins
Methods. Mol. Biol., 1728 :127-135
2018
Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins
Methods. Mol. Biol., 1728 :127-135
2018
Stempel,
E.;
Kaml,
R. F.;
Budisa,
N.;
Kalesse,
M.
Painting argyrins blue: Negishi cross-coupling for synthesis of deep-blue tryptophan analogue β-(1-azulenyl)-l alanine and its incorporation into argyrin C
Bioorg. Med. Chem.
2018
Painting argyrins blue: Negishi cross-coupling for synthesis of deep-blue tryptophan analogue β-(1-azulenyl)-l alanine and its incorporation into argyrin C
Bioorg. Med. Chem.
2018
Baumann,
Tobias;
Exner,
Matthias;
Budisa,
Nediljko
Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis
Advances in biochemical engineering/biotechnology, 162 :1–19
2018
ISSN: 0724-6145
Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis
Advances in biochemical engineering/biotechnology, 162 :1–19
2018
ISSN: 0724-6145
- DOI
- 10.1007/10201637
Kubyshkin,
Vladimir;
Acevedo-Rocha,
Carlos G.;
Budisa,
Nediljko
On universal coding events in protein biogenesis
Biosystems, 164 :16–25
2018
ISSN: 0303-2647
On universal coding events in protein biogenesis
Biosystems, 164 :16–25
2018
ISSN: 0303-2647
Schmidt,
Markus;
Pei,
Lei;
Budisa,
Nediljko
Xenobiology: State-of-the-Art, Ethics, and Philosophy of New-to-Nature Organisms
Advances in biochemical engineering/biotechnology, 162 :301–315
2018
ISSN: 0724-6145
Xenobiology: State-of-the-Art, Ethics, and Philosophy of New-to-Nature Organisms
Advances in biochemical engineering/biotechnology, 162 :301–315
2018
ISSN: 0724-6145
- DOI
- 10.1007/10201614
Biava,
H.;
Schreiber,
T.;
Katz,
S.;
Völler,
J-S.;
Stolarski,
M.;
Schulz,
C.;
Michael,
N.;
Budisa,
N.;
Kozuch,
J.;
Tillmann,
U.;
Hildebrandt,
P.
Long-Range Modulations of Electric Fields in Proteins
J. Phys. Chem. B, B122 (35) :8330-8342
2018
Long-Range Modulations of Electric Fields in Proteins
J. Phys. Chem. B, B122 (35) :8330-8342
2018
Serikawa
;
Spanos
;
Hacht
;
Budisa
;
Rappsilber,
J.;
J.,
Kurreck
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs
Biochimie (144) :169-184
2018
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs
Biochimie (144) :169-184
2018
Nickling,
J. H.;
Baumann,
T.;
Schmitt,
F. J.;
Bartholomae,
M.;
Kuipers,
O. P.;
Friedrich,
T.;
Budisa,
N.
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
J. Vis. Exp., e57551(135)
2018
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
J. Vis. Exp., e57551(135)
2018
- DOI
- 10.3791/57551
Kubyshkin,
V.;
Pridma,
S.;
Budisa,
N.
Comparative effects of trifluoromethyl-and methyl-group substitutions in proline
New. J. Chem., 2018 (42) :13461-13470
2018
Comparative effects of trifluoromethyl-and methyl-group substitutions in proline
New. J. Chem., 2018 (42) :13461-13470
2018
Kubyshkin,
V.;
Budisa,
N.
Exploring hydrophobicity limits of polyproline helix with oligomeric octahydroindole‐2‐carboxylic acid
J. Pept. Sci., 4-5 (24) :e3076
2018
Exploring hydrophobicity limits of polyproline helix with oligomeric octahydroindole‐2‐carboxylic acid
J. Pept. Sci., 4-5 (24) :e3076
2018
Serikawa,
T.;
Spanos,
C.;
vonHacht,
A.;
Budisa,
N.;
Rappsilber,
J.;
Kurreck,
J.
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5′ UTR of tumor-associated mRNAs
Biochimie (144) :169-184
2018
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5′ UTR of tumor-associated mRNAs
Biochimie (144) :169-184
2018
Serikawa,
T.;
Spanos,
C.;
vonHacht,
A.;
Budisa,
N.;
Rappsilber,
J.;
Kurreck,
J.
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5′ UTR of tumor-associated mRNAs
Biochimie, 2018 (144) :169-184
2018
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5′ UTR of tumor-associated mRNAs
Biochimie, 2018 (144) :169-184
2018
Bücher
2006
Budisa,
Nediljko
Engineering the genetic code: Expanding the amino acid repertoire for the design of novel proteins
Herausgeber: Wiley-VCH, Weinheim
2006
Engineering the genetic code: Expanding the amino acid repertoire for the design of novel proteins
Herausgeber: Wiley-VCH, Weinheim
2006
- ISBN
- 3527312439
Patente
2021
Hauf,
Matthias;
Budisa,
Nediljko;
Richter,
Florian;
Baumann,
Tobias;
Schneider,
Tobias
Modified Mussel Proteins, Uses Thereof and Related Compounds
2021
Modified Mussel Proteins, Uses Thereof and Related Compounds
2021
2012
Budisa,
Nediljko;
Wiltschi,
Birgit;
Royter,
Marina;
Antranikian,
Garabed;
Grote,
Ralf
Lipase congeners with enhanced substrate access
2012
Lipase congeners with enhanced substrate access
2012
2009
Budisa,
Nediljko;
Merkel,
Lars;
Cheburkin,
Yuri;
Ullrich,
Axel;
Knyazev,
Piotr;
Wiltschi,
Birgit
Method for the production of proteins with chemically functionalized N-termini
2009
Method for the production of proteins with chemically functionalized N-termini
2009
2004
Budisa,
Nediljko;
Rubini,
Marina;
Huber,
Robert;
Knyazev,
Peter;
Ullrich,
Axel;
Neuefeind,
Torsten
Composition containing peptide that includes amino acid analog, useful for treating e.g. cancer, where the peptide binds to and enters only specific target cells
2004
Composition containing peptide that includes amino acid analog, useful for treating e.g. cancer, where the peptide binds to and enters only specific target cells
2004
2002
Huber,
Robert;
Budisa,
Nediljko;
Moroder,
Luis;
Alefelder,
Stefan;
Bae,
Jae Hyun;
Kaiser,
Jens;
Freye,
Minks Caroline;
Neuefeind,
Torsten
Tryptophan analogs in proteins, peptides and peptidic structures
2002
Tryptophan analogs in proteins, peptides and peptidic structures
2002
Huber,
Robert;
Budisa,
Nediljko;
Moroder,
Luis;
Alefelder,
Stefan;
Bae,
Jae Hyun;
Kaiser,
Jens;
Freye,
Minks Caroline;
Neuefeind,
Torsten
Tryptophan-Analoga in Proteinen, Peptiden und peptidischen Leitstrukturen
2002
Tryptophan-Analoga in Proteinen, Peptiden und peptidischen Leitstrukturen
2002
1998
Nediljko,
Budisa;
Robert,
Huber;
Carolin,
Minks;
Luis,
Moroder
Incorporation of pharmacologically active amino acid analogues into proteins
1998
Incorporation of pharmacologically active amino acid analogues into proteins
1998
Budisa,
Nediljko 82152 Planegg;
Huber,
Robert 82110 Germering;
Minks,
Carolin 82110 Germering;
Moroder,
Luis 82152 Planegg
Inkorporation von pharmakologisch wirksamen Aminosäureanaloga in Proteine
1998
Inkorporation von pharmakologisch wirksamen Aminosäureanaloga in Proteine
1998